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Oxygen use in bacteria


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#1 czernobill

czernobill

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Posted 19 May 2013 - 09:48 AM

Hello,

I used anaerobic bags to test if my bacterial isolates could grow in the absence of oxygen. There was clearly visible, hence little growth. In contrast, when incubating aerobically strong growth is observed. Thus, I would consider the bacteria as facultatively anerobic.

As negative control I used Micrococcus luteus which did not show any growth.

But: My positive control (Staphylococcus saprophyticus, facultatively anerobic) did not grow/very very little in the anaerobic bag. I am confused because I expected them to grow. I do not have a strictly anerobic bacterium. I do not have anaerobic indicator strips.

The questions I would like to ask is whether this method is adequate in judging a species as facultatively anaerobic and why S. saprophyticus did not grow. I inoculated from fresh cultures on appropriate medium.

Furthermore, does anybody know for which purpose oxygen is metabolized in anaerobic spp.? From this metabolization H2O2 is produced which cannot be detoxified. But why do they use oxygen?



Thanks a lot!

Edited by czernobill, 19 May 2013 - 09:54 AM.





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