Western Blot - Quantifying Tumor samples from two different blots
Posted 18 May 2013 - 03:42 PM
I'm new here. And I have a question regarding about quantifying bands on two separate membranes.
So I have a total of 13 protein lysate samples from tumors. Out of those 13, there are 7 control samples, and 6 treated samples.
We only have pre-casted 10 lane gels, so I loaded those 13 samples on two separate gels.
gel 1 has 3 control + 3 treated samples
gel 2 has 4 control + 3 treated samples.
I'd like to ultimately compare the treated samples to control.
After normalizing to the loading control (alpha tubulin), how can i properly combine the numbers from each blot so that I'd have 7 data points for the control group and 6 data points from the treated group? As of now, the trend between the blots between control and treated is consistent (blot 1 and 2 - ~40% decrease of treated compare to control,) but the raw value varies from blot 1 to blot 2, so significance was not reached when entered into graph pad prism.
I'm hesistant to rerun the samples on a gel with more than 13 lanes so i can run all the samples together because we have little of the lysates.
Any help would be appreciated!
- ramankoson likes this
Posted 18 May 2013 - 05:41 PM
Posted 20 May 2013 - 02:26 PM