SH-SY5Y and Poly-L-Lysine
Posted 17 May 2013 - 05:21 PM
I am having problem with neuroblastoma cell line, in particular when I seed the cells on the petri dish, most of them remain in suspension.
Looking on line I see that to overcome this problem some people use poly-l-lysine but I have no idea how to use it,
furthermore, sigma has many type of poly-lysine, could please someone tell me how to use it ? and which kind of poly-lysine is good in term of molecular weight ?
Posted 18 May 2013 - 05:43 PM
Posted 25 May 2013 - 11:41 AM
Posted 14 September 2013 - 05:49 AM
AS I recall using SH-SY5Y before, I did not need to use poly-lysine as long as you use a cell-culture treated flasks. Your cells might have some issues.
Posted 14 September 2013 - 11:42 AM
Plate higher number of cells, and use cell culture treated plates. these help with SHSY5Y cells.