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Immunoprecipitation- Incubation time with beads

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#1 Niaz

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Posted 17 May 2013 - 05:36 AM

Hey guys

In all the protocols I see it is written to incubate the Lysate solution with the antibody overnight and then add the beads next day for an hour or so. I made a mistake and add the beads last night. They are incubated with AB and sample all over the night. Is it going to be a problem? Am I going to have a huge background? should I increase the wash? I pre-cleared the sample with Rabbit serum and beads before.

Please help me
Thanks

#2 bob1

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Posted 17 May 2013 - 04:45 PM

You may have background but if you have pre-cleared the lysate this shouldn't be too much of a problem. The only way to know is to test it...





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