how do I check that my antibody is ChIP grade?
Hi all,
I preforming a ChIP experiment, and to check the efficiency of my antibody, I will make a western blot after IP, but...... It is necesary to reverse the corss linking before?
Thanks!
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1 reply to this topic
#1
valeri_a
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Posted 17 May 2013 - 04:28 AM
Hi all,
I preforming a ChIP experiment, and to check the efficiency of my antibody, I will make a western blot after IP, but...... It is necesary to reverse the corss linking before?
Thanks!
- ramankoson likes this
#2
jerryshelly1
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Excellent
Excellent
Posted 19 May 2013 - 11:37 AM
I just do a titration against my endogenous protein, overexpressing and knockdown lines. I also like to compare my results against any qPCR data I have.
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