Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

How to check an antibody is ChIP grade?

Started by valeri_a, May 17 2013 04:28 AM

ChIP

Please log in to reply

1 reply to this topic

#1 valeri_a

member

Active Members
6 posts

1
Neutral

Posted 17 May 2013 - 04:28 AM

how do I check that my antibody is ChIP grade?

Hi all,

I preforming a ChIP experiment, and to check the efficiency of my antibody, I will make a western blot after IP, but...... It is necesary to reverse the corss linking before?

Thanks!

ramankoson likes this

#2 jerryshelly1

Veteran

Global Moderators
309 posts

29
Excellent

Posted 19 May 2013 - 11:37 AM

I just do a titration against my endogenous protein, overexpressing and knockdown lines. I also like to compare my results against any qPCR data I have.

Back to -Genetics and Epigenetics-

Also tagged with one or more of these keywords: ChIP

Protocols and Techniques Forums → ChIP and Next Generation Sequencing → How to concentrate chromatin prior to IP? Started by Brenna, 19 Mar 2014 ChIP, chromatin shearing	0 replies 131 views	Brenna 19 Mar 2014
Protocols and Techniques Forums → ChIP and Next Generation Sequencing → double crosslinking in PBS or medium? Started by Txaio83, 19 Mar 2014 chip, double crosslinking, PBS	2 replies 112 views	Txaio83 20 Mar 2014
Protocols and Techniques Forums → Molecular Cloning → Which epitope tag to use for ChIP? Started by Epigeneticist, 09 Jan 2014 Epitope Tag, Cloning, Chip and 1 more...	1 reply 161 views	Epigeneticist 13 Jan 2014
Protocols and Techniques Forums → ChIP and Next Generation Sequencing → Comprehensive List of transcription factor binding site databases based on ChIP- Started by zhxq09, 18 Nov 2013 chip, ChIP-Seq, NGS, sequencing and 1 more...	0 replies 323 views	zhxq09 18 Nov 2013
Protocols and Techniques Forums → Molecular Biology → Nuclei fixation using formaldehyde Started by Kriti Khandelwal, 16 Oct 2013 formaldehye, 4C, ChIP, fixation	0 replies 366 views	Kriti Khandelwal 16 Oct 2013