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How to check an antibody is ChIP grade?

Started by valeri_a, May 17 2013 04:28 AM

ChIP

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#1 valeri_a

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Posted 17 May 2013 - 04:28 AM

how do I check that my antibody is ChIP grade?

Hi all,

I preforming a ChIP experiment, and to check the efficiency of my antibody, I will make a western blot after IP, but...... It is necesary to reverse the corss linking before?

Thanks!

ramankoson likes this

#2 jerryshelly1

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Excellent

Posted 19 May 2013 - 11:37 AM

I just do a titration against my endogenous protein, overexpressing and knockdown lines. I also like to compare my results against any qPCR data I have.

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