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How to check an antibody is ChIP grade?

ChIP

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#1 valeri_a

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Posted 17 May 2013 - 04:28 AM

how do I check that my antibody is ChIP grade?


Hi all,

I preforming a ChIP experiment, and to check the efficiency of my antibody, I will make a western blot after IP, but...... It is necesary to reverse the corss linking before?


Thanks!

#2 jerryshelly1

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Posted 19 May 2013 - 11:37 AM

I just do a titration against my endogenous protein, overexpressing and knockdown lines. I also like to compare my results against any qPCR data I have.





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