What housekeeper to use with HEK cells?
Posted 17 May 2013 - 03:39 AM
I am working with HEK293 cells and tried to check my RNA integration by using usual housekeepers (EF-1a, GAPDH, SDHD) but apparently they are not able to bind to this template, although in silico analyses revealed that they should. I tried them with quantitative PCR as well as with conventional RT-PCR with the same results.
I would be really happy for any suggestions.
Thanks a lot,
Posted 17 May 2013 - 04:51 PM
Posted 18 May 2013 - 08:00 AM
Btw: are there possibly any differences between transfected vs. non-transfected HEK cells in expressing these genes?
Edited by Stephp, 18 May 2013 - 08:01 AM.
Posted 18 May 2013 - 05:44 PM