Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Housekeeper for HEK cells?

HEK cells house keeper PCR qPCR

  • Please log in to reply
1 reply to this topic

#1 Stephp

Stephp

    member

  • Members
  • Pip
  • 3 posts
1
Neutral

Posted 17 May 2013 - 03:38 AM

Hello everybody,

I am working with HEK293 cells and tried to check my RNA integration by using usual housekeepers (EF-1a, GAPDH, SDHD) but apparently they are not able to bind to this template, although in silico analyses revealed that they should. I tried them with quantitative PCR as well as with conventional RT-PCR with the same results.

I would be really happy for any suggestions.

Thanks a lot,

Best
Stephanie

#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,113 posts
59
Excellent

Posted 18 May 2013 - 09:57 AM

That's strange. You gapdh must give a sharp band if your cdna is synthesized properly.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.