Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells)NSC34 Collagen Gelatin
Posted 16 May 2013 - 08:52 PM
My supervisor wants quantitative confirmation that my cells are doing better on poly-L or collagen than gelatine before he coughs up $284 for pure type I rat tail collagen (from Sigma) as he does not have the funds. I wanted to know whether or not that was possible? By that I mean, I'm not sure whether that sort of data would be quantitative (e.g. cell density) or qualitative (cell adherence) because NSC34s will happily proliferate whether or not they are properly adhered to the surface (they are a reasonably aggressive proliferating cell line).
Please let me know what you think. Is it more of a qualitative or quantitative measure?
And which substrate would be best? Gelatin (which I've heard dissolves in modern day media over a period of 1 week) or collagen (at 0.01%) or poly-L (at 0.01%)?
Posted 30 May 2013 - 02:20 PM
A possible quantitative measurement would be a viability assay - are the cells more viable if on collagen / poly-lysine ?
Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.