Posted 14 May 2013 - 06:45 AM
I'm doing my phagocytosis assay based on a research paper which stated "before commencing assay, cells were opsonized with 10ug/ml monoclonal antibody". That's pretty vague to me becaused I dont know for sure how many cells would be opsonized by that amount of antibody (ie 10^5 cells/ml opsonized by 10ug/ml or what?). Are there any ways that I can determine the exact (or relative) number of cells that were opsonized by a particular amount of antibody?
I'm rather new at these stuffs so any helps would be very much appreciated!
Posted 14 May 2013 - 08:55 AM
Posted 14 May 2013 - 10:48 PM
I thinking about a setting up a new serum bacteriocidal assay (opsonizing using different concentrations of antibody and incubate with complements and observe complement killing capacity) but wonder if there are any simpler ways?