Hey guys,
I wanna extract membrane proteins (cell membrane, mitochondrial,& nuclear membrane) as per the protocol i have to centrifuge the cell homogenate at 100000g for 10min. since i dnt have a high speed centrifuge can i centrifuge it at 50,000g increasing the time. will i get the membrane proteins separated?
Roshan
Submit your paper to J Biol Methods today!
2 replies to this topic
#2
mdfenko
-
- Active Members
-
- 2,843 posts
an elder
145
Excellent
Excellent
Posted 14 May 2013 - 06:02 AM
it should work if you at least double the time
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
labtastic
-
- Active Members
-
- 84 posts
Enthusiast
3
Neutral
Neutral
Posted 14 May 2013 - 09:38 AM
Our lab routinely uses 40-50,000 x g for 1hr to spin out membranes and collect the soluble fraction for subsequent protein purification.
