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Membrane protein extraction


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2 replies to this topic

#1 Roshan

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Posted 13 May 2013 - 07:44 PM

Hey guys,
I wanna extract membrane proteins (cell membrane, mitochondrial,& nuclear membrane) as per the protocol i have to centrifuge the cell homogenate at 100000g for 10min. since i dnt have a high speed centrifuge can i centrifuge it at 50,000g increasing the time. will i get the membrane proteins separated?

Roshan

#2 mdfenko

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Posted 14 May 2013 - 06:02 AM

it should work if you at least double the time
talent does what it can
genius does what it must
i do what i get paid to do

#3 labtastic

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Posted 14 May 2013 - 09:38 AM

Our lab routinely uses 40-50,000 x g for 1hr to spin out membranes and collect the soluble fraction for subsequent protein purification.




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