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Lysis buffer for white matter

western blot white matter myelin

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#1 Hilary57

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Posted 13 May 2013 - 11:16 AM

Hi

I am a newbie to electrophoresis and western blotting. I need to measure white matter proteins (MBP, PLP, etc) in corpus callosum samples. Is RIPA an appropriate buffer for tissue that is so high in lipids, or should I use something else? I can't seem to find any consistency in the literature.

Thanks!

Hilary

#2 Curtis

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Posted 15 May 2013 - 09:33 PM

if you need total lysate then RIPA is a very strong lysis buffer. unless you need other cellular fractions, then you need to use milder lysis buffers.





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