No replies to this topic

[harry348]

I have a problem in calculating a dilution factor for my elisa samples.hope you guys can help me out.
This is what i did

I have two samples(A & with concentration of sample A 2.93ug/ul (39.3ul sampl + 60.1ul diluent ) and sample B 3.48ug/ul (33.5ul sample + 66.5ul diluent), i diluted these two sample to a concentration of 1.17ug/ul to a final volume of 100ul.these samples are done in duplicates

After elisa based on the standard curve(spline curve) i got a concentrations of sample A 0.423ng/ml and 1.285ng/ml, and for sample B 1.14ng/ml and 1.67ng/ml.

Now i want to know what is the concentration of my protein sample before dilution.

Thanks in advance

Edited by harry348, 12 May 2013 - 07:01 AM.