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Native gel electrophoresis: Can it tell if a protein is forming dimer?


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#1 Bioscientist

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Posted 08 May 2013 - 11:22 AM

Hi there

I have a protein and I want to determine if it forms homodimer or not. If I run it by Native gel electrophoresis will I be able to see if it forms dimer or not? If so how will it look in the native gel?

Thanks.

Bioscientist.

#2 bob1

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Posted 08 May 2013 - 01:21 PM

You should be able to by running some denatured protein alongside so that you will see the monomer and look for a doubled size band. You could also transfer and then use an antibody to detect.




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