Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primer Efficiency across runs

PCR Primer RT-PCR Efficiency Control

  • Please log in to reply
1 reply to this topic

#1 Joe101

Joe101

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 08 May 2013 - 10:24 AM

Hi,

When running serial dilutions for testing primer efficiency, in this case for use with the Pfaffl method, do they need to be run on the same plate as the samples you are testing? I had allways thought of primer efficiency beign a property of the primer design, but I guess this must interact with running conditions to a certain extent.

Thanks,
Joe

Edited by Joe101, 08 May 2013 - 10:25 AM.


#2 Tabaluga

Tabaluga

    And we're back to square one

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 316 posts
43
Excellent

Posted 30 May 2013 - 12:52 PM

I think they should be run on the same plate.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.