Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Accidentally left some serum out overnight at room temp....


  • Please log in to reply
3 replies to this topic

#1 jmleal

jmleal

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 May 2013 - 09:07 AM

I know, bonehead mistake! I think I already know the answer to this, but it would be helpful for some feedback. I ran an ELISA on some serum obtained from immunized mice, and I left it out at room temp. I'm guessing this serum can't be used for any future experiments, but I'm not sure. Its not the end of the world, but I was hoping to run a follow up experiment. Thanks for the help.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,833 posts
415
Excellent

Posted 08 May 2013 - 01:25 PM

Yeah, not a good idea, many antibodies are pretty unstable. General rule is "if in doubt, throw it out" This applies pretty much anywhere in lab work, as if you start doubting the results you got because you know you made a mistake somewhere, you are going to have to repeat it anyway with the good reagent etc, so throw out the bad one and do it again...

#3 jmleal

jmleal

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 08 May 2013 - 02:30 PM

Good point. Thanks for the advice.

#4 Missle

Missle

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 132 posts
18
Good

Posted 10 May 2013 - 06:14 AM

When in doubt throw it out is always good advice and I would be concerned about contamination leaving serum at RT overnight. However, the antibody would most likely be fine and stable. Most antibodies are very stable in serum and they are fine at warmer temperatures (as it is their natural environment). The stability is more of a concern once it has been purified and even then there is probably less concern about a 18hr at room temp then there would be about a freeze-thaw cycle.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.