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elisa OD calculation


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#1 tulip

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Posted 08 May 2013 - 08:47 AM

Hi,

I need to measure TNFalpha secretion in the cell supernatent. The final volume is 0.5ml. The OD of the standards is pg/ml so do I need to halve the OD's of the test samples as the final volume is 0.5 ml.

Thanks.

#2 bob1

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Posted 08 May 2013 - 01:29 PM

No, absorbance is a function the pathlength of the light though the sample in the well (see Beer-Lambert law - so if you loaded 100 ul into each well for both standards and samples then there isn't a problem, results should be directly convertable.

#3 tulip

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Posted 09 May 2013 - 03:25 PM

Thanks a lot.




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