Hi,
I need to measure TNFalpha secretion in the cell supernatent. The final volume is 0.5ml. The OD of the standards is pg/ml so do I need to halve the OD's of the test samples as the final volume is 0.5 ml.
Thanks.
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2 replies to this topic
#2
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 08 May 2013 - 01:29 PM
No, absorbance is a function the pathlength of the light though the sample in the well (see Beer-Lambert law - so if you loaded 100 ul into each well for both standards and samples then there isn't a problem, results should be directly convertable.
