Question about pipet tips for PCR and rtPCR
Posted 08 May 2013 - 06:18 AM
We have been using the Fisher Sure One tips for doing our PCR's and rt PCR reactions. This summer there are going to be quite a bit of students in our lab working on PCR. In the past we have isolated RNA from our samples and then kept it in the -80 degree freezer, and thawed the sample when we needed some.
This summer we are planning on extracting the RNA, converting all to cDNA and freezing this. I understand that with RNA we need to use the RNase free pipet tips like the Sure Ones. However, once we are using cDNA is this necessary or can we use the general lab stock pipet tips?
Thanks in advance.
Posted 08 May 2013 - 01:31 PM
Posted 09 May 2013 - 05:22 AM
Posted 09 May 2013 - 07:55 PM