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UNG in PCR

Started by xxcici, May 05 2013 07:19 AM

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#1 xxcici

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Posted 05 May 2013 - 07:19 AM

Why UNG is used in PCR step? Can some body explain it simply to me? Thanks.

#2 bob1

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Posted 05 May 2013 - 12:15 PM

Basically it is a dTTP analogue that gets incorporated during the PCR and can be used to prevent carry-over.  See here
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