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Allosteric regulation


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#1 luxzo

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Posted 04 May 2013 - 09:07 AM

How can we determine the allosteric regulation of an enzyme experimentally?

#2 mdfenko

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Posted 06 May 2013 - 04:15 AM

the short answer is: assay the enzyme

longer: if positive substrate cooperativity, then substrate saturation kinetics will show a sigmoidal curve (as opposed to hyperbolic)

if cofactor then assay with and without said cofactor

if negative cooperativity, then inhibition assays
talent does what it can
genius does what it must
i do what i get paid to do




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