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Soluble protien of GST

soluble GST protein

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2 replies to this topic

#1 seeking_2012

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Posted 02 May 2013 - 05:55 PM

Hi there,
I have cloned my gene of interest into pGEX-2T vector. I have indue it with IPTG for different concentration and different temprature different competant cells (my cell BL21 ) but usually it become insoluble. I am using thermo kit for purification, however I used Sarkosyl but i have got promssing but protien.
Please If you have any method to soluble my protien without effect the protien I will use it for Histone Methyltransferase - Assay

#2 seeking_2012

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Posted 06 May 2013 - 02:03 AM

any help

#3 mdfenko

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Posted 06 May 2013 - 04:33 AM

you can solubilize it in urea and then refold after purification by slowly dialyzing away the urea.
talent does what it can
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