restain secondary antibody
Posted 02 May 2013 - 12:37 PM
I am doing the immunohistochemistry on the tissues, in the gel about 150um (cut with vibratome).
I made a mistake in my secondary antibody, instead of using secondary anti-rabbit green + secondary anti-mouse red; i used secondary anti-rabbit red and secondary anti-mouse green . I did a double staining for my primary antibodies, one is against rabbit and the other is mouse.
Is there a way to remove the secondary antibody and restain my tissues slice?
Posted 02 May 2013 - 07:59 PM
Posted 03 May 2013 - 06:18 AM
The problem i am facing now is, i try to identify GFP staining in my tissues and one of the primary antibody is against the GFP and i am supposed to stain the GFP with secondary in green to enhance the GFP signalling but i made a silly mistake, using red for the GFP antibody .
Posted 04 May 2013 - 01:08 AM
Posted 09 May 2013 - 12:24 AM
May i know what is the commonly used stripping protocols...at least there's still some slight hope to try...
Posted 09 May 2013 - 12:49 AM