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Dual Luciferase Assay Buffers

luciferin coelenterazine luciferase

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#1 VitorMC

VitorMC

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Posted 02 May 2013 - 07:12 AM

Hello,

I am starting to perform a dual luciferase assay in cells transfected with a plasmid containing my interest 3UTR bound to a firefly luciferase, and also a renilla luciferase gene with a plasmid overexpressing a microrna. I know that there are several commercially available kits for the determination of dual luciferase activities. However i would like to do the solutions of buffers, d-luciferin and coelenterazine by my own. does anyone have a protocol for the determination of firefly and renilla luciferases? I already have a protocol with buffers compositions for firefly luminescence detection, however i am not sure if this buffers will be compatible with coelenterazine and i don't want to risk buying it and than it not working.

Thank you very much for your attention

Regards

VitorMC





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