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What's the chemistry behind flocculation when Qiagen P2 is mixed with N3


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#1 Ahrenhase

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Posted 01 May 2013 - 10:12 AM

P2 (or the lysis buffer) is 200mM NaOH and 1% SDS. N3 (or neutralization buffer) is 4.2M GuHCl and 0.9M potassium acetate. What is floccing out? Is it the SDS which is bound to proteins?

#2 phage434

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Posted 01 May 2013 - 11:13 AM

KDS is much less soluble than SDS, so it precipitates. The main role is to physically capture the genomic DNA to reduce gDNA contamination in the final eluted product.




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