P2 (or the lysis buffer) is 200mM NaOH and 1% SDS. N3 (or neutralization buffer) is 4.2M GuHCl and 0.9M potassium acetate. What is floccing out? Is it the SDS which is bound to proteins?
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What's the chemistry behind flocculation when Qiagen P2 is mixed with N3
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