[font=宋]I have performed an EMSA recently and have observed nice binding to my probe. When I added antibody to try to identify the protein binding the probe, I saw a decrease in band intensity, which others in my lab tell me is indicative of the antibody binding the protein:probe complex, however I do not see a supershift of the complex. I have tried adding different amounts of antibody, but I am still unable to see a supershift.
This antibody has been used to EMSA before . How do I optimise the assay for the supershift? [/font]
[font=宋]Thanks in advance!!![/font]