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pMALc2x transformation into MG1655


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#1 danaida9

danaida9

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Posted 28 April 2013 - 08:28 AM



I want to transform E.coli strain MG1655 with my plasmid (pMALc-2x derivative, MBP fusion). Unfortunately I cannot get any clones. I can transform those cells with different plasmid (so they are competent) and I can transform my plasmid to different strain. It's just this combination which doesn't work. It's not because of my gene - I don't get any clones even if I try with empty plasmid. Does anyone know what could be the reason? Thanks a lot!




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