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Sensitive, Consistent and Reproducible Method(s) for Extraction of gDNA from Low

Blood gDNA Extraction Consistency Low Volume

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#1 Fluoresce

Fluoresce

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Posted 27 April 2013 - 06:35 PM

Hello all,
Have tried to extract gDNA (frozen whole blood) (less than 300 ul).  I have had not much luck to have a sensitive, consitent/reproducible extraction with methods such as salting out or magnetic beads.  It is critical to use low volumes for my purposes.  Can anyone suggest another method such as 96 well format extraction that has been tested and showed good results as far as reprodicibility/consistency? I'd really apprecite your inputs.
I can also use plasma (super low volume).

Thanks,
FL





Also tagged with one or more of these keywords: Blood, gDNA, Extraction, Consistency, Low Volume

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