hello, right now, i already finished transform and co-transform my recombinant dna with supporters plasmid. the problem is when i doing sequencing, there an insertion of nucleotide occurred. this is impossible because my recombinant dna is a wild type dna..any suggestion or idea to solve this problem?
right nnow, i just redo sequencing cycle.
last one, what the software or website to generate the recombinant dna map? i used once and totally forgot..
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