urgent: need help with qPCR statistics
Posted 26 April 2013 - 08:06 AM
I don't have standard curve when I did qPCR. But in each plate I included one standard bacteria strain gene (in triplicate). I want to ask how should I analyze the data. Should I simply compare Ct value by nonparametric analysis? Or shoud I do some others (like delta CT?)?
I see one paper compare gene difference in patients and healthy control by "fold change". But I don't know what they used to compare. Just compare Ct?
I need the results be analyzed by the end of the day. Would you please help me with that?
Thanks a lotu
Posted 26 April 2013 - 08:30 AM
what is your internal control - the control that tells you a difference in the Ct of bacteria 16S comes from a difference in infection or in unequal starting RNA? If you have included an internal control, then you use delta Ct to obtain means for each group of patients, and then use ANOVA to compare among groups.
Posted 26 April 2013 - 09:37 AM