Is there a maximum blocking time?
Posted 26 April 2013 - 01:52 AM
I prepared a 96 well plate with capture AB and over night incubation @ 4°C.
I washed twice and put PBS+10%FCS in each well to block. Unfortunately I cannot continue work today, so is it possible to leave the blocking solution on the wells @ 4°C for 2 days or might this be detrimental? As I already incubated with capture AB I don't want to discard the plate...
What can I do?
Posted 26 April 2013 - 02:00 AM
I have tried it before - however longer, for about 3 days - and have gotten a lot of background and artificial signaling.
If you use this plate, make sure that you have a negative control and a blank well.
Posted 26 April 2013 - 05:33 AM
I guess the real question is how precious are the rest of the samples/reagents for the rest of your assay? If you can't risk repeating the entire assay then discard them now......