Hi
I am working on the C. elegans neurodegenerative model. I am doing drug analysis against them. When I do do its western blot by using sigma monoclonal antibody at different conc. I don't get my desired bands at 4kDA. If I increase the conc of primary antibody, it gives a alot of non specific bands.
If have also used 4G8 antibody but no luck.
Can any body help me in this? Your suggestions are highly welcomed
1
Western Blotting of Amyloid Beta
Started by Waqar Ahmad, Apr 25 2013 02:22 AM
Amyloid Beta C. elegans Western Blot
1 reply to this topic
#2
mdfenko
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Excellent
Excellent
Posted 25 April 2013 - 05:08 AM
have you followed the procedures posted by covance (this link will download a pdf of the protocol)?
4g8 (and 6e10) were developed at my institute. i've used it and found it to be finicky about the conditions used (although we used it with a higher salt concentration (500mM) than covance specifies to improve specificity).
covance also gives specific information on how much to use.
4g8 (and 6e10) were developed at my institute. i've used it and found it to be finicky about the conditions used (although we used it with a higher salt concentration (500mM) than covance specifies to improve specificity).
covance also gives specific information on how much to use.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
