1 reply to this topic

[Waqar Ahmad]

Hi
I am working on the C. elegans neurodegenerative model. I am doing drug analysis against them. When I do do its western blot by using sigma monoclonal antibody at different conc. I don't get my desired bands at 4kDA. If I increase the conc of primary antibody, it gives a alot of non specific bands.
If have also used 4G8 antibody but no luck.
Can any body help me in this? Your suggestions are highly welcomed

[mdfenko]

have you followed the procedures posted by covance (this link will download a pdf of the protocol)?

4g8 (and 6e10) were developed at my institute. i've used it and found it to be finicky about the conditions used (although we used it with a higher salt concentration (500mM) than covance specifies to improve specificity).

covance also gives specific information on how much to use.