Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

5'-azacytidine treatment

how often medium change

  • Please log in to reply
1 reply to this topic

#1 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 196 posts
2
Neutral

Posted 24 April 2013 - 11:57 PM

I'm treating my cell lines with 5ázacytidine to check for methylation. I'm planning to treat my cell lines with 5ázacytidine for 5 days. Do I have to replace the medium and drug every day or can I do it alternatively?

Álso, Ive made my stock solution of 5ázacytidine (Sigma, A2385) in DMSO and frozen the aliquots in
-80 degrees.

Thank you

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 25 April 2013 - 11:07 PM

The treatment protocol may vary with different cell lines. Since aza-cytindine is very toxic to cells, to achieve demethylation without killing all cells, for many cell lines, you have to treat the cells non-consecutively, which means treating cells every other day and change to fresh medium in between. Some cell lines need longer treatment time i.g. two weeks.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.