Freezing Cells For Use In Assays
Posted 24 April 2013 - 06:55 AM
I am looking for a protocol that allows me to freeze down cells in batches which i can then store in the -80 for use in assays over a period of a few months. I have used cells like this previously. On the day of experiment I took them out of the -80 and quickly thawed them, resuspend (to remove DMSO) and left for 30 mins; they have worked great. Someone else used to make the cells but now I have to, and I dont even know what the method is called to search for it!
Posted 24 April 2013 - 01:31 PM
Which type of cells are they ?
Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.
Posted 25 April 2013 - 01:39 AM