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Best assays for LIF and bFGF Activity?

LIF bFGF pluripotency proliferation signaling

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#1 doxorubicin

doxorubicin

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Posted 24 April 2013 - 04:02 AM

We're trying to set up some routine assays to test new batches of LIF and bFGF we receive before using them for stem cell tissue culture. Does anyone have any good recommendations for the most telling assays? Company datasheets show bFGF assays (3H-thymidine uptake by BAF3 cells overexpressing FGF-receptor, CREB phosphorylation in NIH/3T3 cells, or proliferation of NIH/3T3 cells) and LIF assays (differentiation of M1 mouse myeloid cells in soft agar, STAT3 phosphorylation in TF-1 cells, and proliferation of TF-1 cells). Does anyone know which assays are the best?





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