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FAM and VIC S-curves

qPCR FAM VIC DNA RNA

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#1 squallweathered

squallweathered

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Posted 23 April 2013 - 07:35 AM

Hi all,

When using a VIC labelled TaqMAN probe in a singleplex qPCR assay of a DNA target on an AB7900, the S-curves come up in both VIC (expected!) and FAM. I realise that this is to be expected due to the spectral overlap.

However, when a similar assay is carried out but the original source material was RNA based (and converted to cDNA), S-curves are only generated in the VIC channel with no "bleed" into FAM at all.

Can anyone offer an explanation of why this would be?






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