May i ask of after i stained the fixed cell on dish, using oil red o on the whole dish, can I still do the IF on the cells which are not stained with Oil Red O...
Is the condition suitable for IF...
Thank you
Submit your paper to J Biol Methods today!
2 replies to this topic
#1
wdyeo
-
- Active Members
-
- 54 posts
Enthusiast
4
Neutral
Neutral
Posted 23 April 2013 - 02:30 AM
Dear all,
May i ask of after i stained the fixed cell on dish, using oil red o on the whole dish, can I still do the IF on the cells which are not stained with Oil Red O...
Is the condition suitable for IF...
Thank you
May i ask of after i stained the fixed cell on dish, using oil red o on the whole dish, can I still do the IF on the cells which are not stained with Oil Red O...
Is the condition suitable for IF...
Thank you
#2
bob1
-
- Global Moderators
-
- 5,904 posts
Thelymitra pulchella
425
Excellent
Excellent
Posted 23 April 2013 - 12:41 PM
So long as you use a wavelength that doesn't cause the oil red to fluoresce you should be fine I think, though I have never tried it.
#3
wdyeo
-
- Active Members
-
- 54 posts
Enthusiast
4
Neutral
Neutral
Posted 24 April 2013 - 02:59 AM
Hi bob1,
Thanks alot for the point.
Have a nice day
Thanks alot for the point.
Have a nice day
