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Oil red o - IF


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#1 wdyeo

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Posted 23 April 2013 - 02:30 AM

Dear all,
May i ask of after i stained the fixed cell on dish, using oil red o on the whole dish, can I still do the IF on the cells which are not stained with Oil Red O...
Is the condition suitable for IF...
Thank you :D

#2 bob1

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Posted 23 April 2013 - 12:41 PM

So long as you use a wavelength that doesn't cause the oil red to fluoresce you should be fine I think, though I have never tried it.

#3 wdyeo

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Posted 24 April 2013 - 02:59 AM

Hi bob1,
Thanks alot for the point.
Have a nice day :D




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