i am currently looking at the expression levels of a protein with an eGFP tag in HEK 293 cells via FACS analysis. For my next round of measurements i need 8h, 16h and 24h after induction.
Here is my Problem: in my experiments before (24h and 48h) i timed it in a way that i could do the FACS immediatly. But with these shorter times i will not be able to measure them immediatly.
So i am currently looking for a way to store my HEK293 cells for about two days that does not interfere with the FACS analysis and stops the cells from expressing any of my eGFP tagged protein.
Can i store them at 4°C or is -20°C preferable. Do i need to fixate them?
Any help is greatly appreciated.
Edited by Carnivora, 23 April 2013 - 01:20 AM.