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quick change mutagenesis......

Help me out plz.........

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Posted 29 April 2013 - 03:17 AM

so no issues with the taq.......so the purity of your prep might be the possible concern here. so reprecipitate your plasmid and digest again, or go with miniprep kits for isolation and proceed.

about the retardation on gel, not always you see the difference between the empty and insert containing plasmid, so need'nt worry abt that.

I always had an alternate hypothesis....

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