so no issues with the taq.......so the purity of your prep might be the possible concern here. so reprecipitate your plasmid and digest again, or go with miniprep kits for isolation and proceed.
about the retardation on gel, not always you see the difference between the empty and insert containing plasmid, so need'nt worry abt that.
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quick change mutagenesis......Help me out plz.........
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