Any tips on how to avoid lifting cells in 96well plates
Posted 20 April 2013 - 03:59 AM
I work with MTS assay for drug inhibition. I did a practise run before the real experiment and tried dosing the drug each day. Whilst changing the media I realized that some of my adherent cells tear off and form a scaffold and some get lost. A549 seems fine and does not get dislodged but some other cancer cell lines do. Has anyone experienced this and would like to give some tips? I'd appreciate it.
Posted 20 April 2013 - 07:54 AM
Posted 23 April 2013 - 01:11 PM
Posted 24 April 2013 - 03:28 AM
Thanks for the responses, I will try inverting them and patting on absorbent paper. Also putting the medium in slowly.