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Concanavalin A induced PBMC proliferation


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#1 neha1905

neha1905

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Posted 18 April 2013 - 09:06 PM

I am trying to study the proliferation of PBMC in the presence of immunogenic peptides using MTT assay.I am using Concanavalin A (Con A) as a positive control for my studies. But the issue is that Con A is not showing any proliferation.
Media used is RPMI 1640 supplimented with 10%FBS
MTT ( sigma) in PBS
ConA (Sigma) in PBS

Suggestions required.




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