Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Concanavalin A induced PBMC proliferation

  • Please log in to reply
No replies to this topic

#1 neha1905



  • Members
  • Pip
  • 1 posts

Posted 18 April 2013 - 09:06 PM

I am trying to study the proliferation of PBMC in the presence of immunogenic peptides using MTT assay.I am using Concanavalin A (Con A) as a positive control for my studies. But the issue is that Con A is not showing any proliferation.
Media used is RPMI 1640 supplimented with 10%FBS
MTT ( sigma) in PBS
ConA (Sigma) in PBS

Suggestions required.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.