Dear All,
I would like to reach a OD600 greater than 50 in flask and 100 in Fermenter. Do someone knows a good media beside LB, TB or SB?
Thanks in advance
All the Best
Submit your paper to J Biol Methods today!
2 replies to this topic
#2
bob1
-
- Global Moderators
-
- 5,507 posts
Thelymitra pulchella
370
Excellent
Excellent
Posted 18 April 2013 - 02:10 PM
For which bugs?
#3
Pangea
-
- Active Members
-
- 97 posts
Enthusiast
7
Neutral
Neutral
Posted 24 April 2013 - 10:33 PM
BL21 DE3 or Origami ? But if you pass me all formulation what you it would be great. I think i will try auto-induction media with 2 mM MgSO4 or higher. Some Trace Elements. I know LB, TB, SB, M9 etc. but some how people have good media formulation. Actually, I get with 2xTB at 37 C and 0.5mM İPTG an OD600 of 14 in a shaking flask (100 ml/500ml). Any suggestions?
