tag for protein purification
Posted 17 April 2013 - 06:05 PM
I am now planning an expression of recombinant protein and I want to use HA tag for protein purification. But my supervisor pointed out that for a large scale purification I will need cheaper tag (HA tag requires using of antibodies).
Can anyone suggest a cheaper tag?
Another question, does it make sense to add a restriction sites at the ends of the tag so the expression cassette could be modified (tag removed/changed)?
Thanks for the help!
Posted 22 April 2013 - 09:53 AM
Posted 06 May 2013 - 10:46 PM
Posted 14 May 2013 - 09:42 AM
Posted 21 May 2013 - 09:40 PM
Posted 22 May 2013 - 05:07 AM
genius does what it must
i do what i get paid to do
Posted 22 May 2013 - 05:32 AM
Regardless, if that is the case, then a strep tag should work just fine. The other option is a cleavable GST tag, which you can purify using glutathione beads (edit: mdfanko just beat me to it ).
Or lastly, you could go "old school" and purify it without a tag. Finding the right purification scheme could take some time but is very much doable, especially if the protein overexpresses reasonably well.
Edited by labtastic, 22 May 2013 - 05:33 AM.