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Incubate tubes on ice vs in the refrigerator


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#1 bjk1985

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Posted 17 April 2013 - 05:33 PM

Maybe a dumb Q... but I've been doing a protocol for DNA isolation from soil that has a couple of precipitation steps that, after addition of solution and brief vortexing, says to incubate at 4 C for 5 min. I've just been sticking them in ice (a bucket of ice that itself is at RT). Can you see any problems in doing this?

#2 phage434

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Posted 17 April 2013 - 05:50 PM

Well, it will be at 0C rather than 4C, but that's probably better. Additionally, the ice bucket (especially if it is wet ice) will transfer heat much more quickly than putting a tube in the refrigerator.

#3 bjk1985

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Posted 25 April 2013 - 05:13 PM

Yeah, I did it to get the temp (inside the tubes) down faster since it only calls for 5 min. I didn't think it would be an issue (that somehow resulted in added contamination, particularly humic acids in this case), but there of couse always could be a mechanism that I'm not aware of that could occur if temp of solution got below 4 C.




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