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Autoconversion of photoconvertable fluorophore

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#1 Ahrenhase



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Posted 16 April 2013 - 11:59 AM

I'm working with the photoconvertable fluorophore, Kaede, and in neurons it seems to have a certain level of autoconversion. I don't think this is autofluorescence or an issue of overexposure because my controls don't autofluoresce red and I'm using the same exposure time as I do in other cell types (also transfected with Kaede), respectively.

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