problems with compensation PE & FITC
Posted 16 April 2013 - 02:09 AM
I use flow cytometer with two lasers (five filters).
Did anyone have similar problem? I already did some troubleshooting and I am about to try again but will welcome any suggestions,thanks
Posted 08 July 2013 - 12:51 PM
Let's say we are compensating for PE. In our compensation tube, we will have CD4+ cells, and ideally some CD4- cells. If we add CD4-PE antibody to the tube, two populations will appear along that PE axis. However, the two populations should be equally negative in every other channel Remember, we only added CD4-PE to that tube; not any other antibodies.
So, if we plot CD4-PE on the y-axis and another fluorochrome (FITC, in your case) on the x-axis, we should see two populations:
Hope that helps!
- murusa likes this
Posted 10 September 2013 - 10:58 PM
thank you, JMoynihan! the excellent explanation? very clear even without pictures of plots!