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Real time PCR statistics


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#1 mnqcljsm

mnqcljsm

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Posted 16 April 2013 - 01:57 AM

Dear all,

Has anyone got any bright ideas on this subject?

I have measured the relative expression of a gene between 2 populations, and repeated this on 3 different days using delta delta Ct

What i want to know is whether or not the difference in transcript abundance between the populations is significantly different.

I can't simply compare the 2 groups of 3 delta Ct values because the work for each day was carried out independently (so different amounts of starting RNA, etc) but this doesn't matter because it is the relative values (i.e. the delta delta Ct) for each day that are important.

Has anyone had any experience with this? Have looked into REST, etc but seems overcomplicated. There must be a simple test to determine whether relative expression values (expression ratios) are significantly different from zero?

Any help would be appreciated and would make sure i pay it forward

Thanks




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