Hello, I am working with CHO cells expressing the hM2 receptor. Usually, I perform my experiments with this buffer: 12.5 mM HEPES, 12.5 mM MgCl2; 125 mM NaCl. For my next experiments I need a sodium-free buffer, pH around 7.4
Could anyone suggest me a similar buffer, with the same ionic strenght, but sodium-free?
Thank you!
2
Possible substitutions of HEPES buffer with sodium-free buffers
Started by Annasae, Apr 16 2013 01:43 AM
HEPES sodium-free buffers CHO cells
2 replies to this topic
#2
mdfenko
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Excellent
Excellent
Posted 16 April 2013 - 03:46 AM
you could use potassium chloride instead of sodium chloride and potassium hydroxide to adjust the hepes buffer (unless it will cause problems with the cells).
talent does what it can
genius does what it must
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genius does what it must
i do what i get paid to do
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