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Possible substitutions of HEPES buffer with sodium-free buffers

HEPES sodium-free buffers CHO cells

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#1 Annasae

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Posted 16 April 2013 - 01:43 AM

Hello, I am working with CHO cells expressing the hM2 receptor. Usually, I perform my experiments with this buffer: 12.5 mM HEPES, 12.5 mM MgCl2; 125 mM NaCl. For my next experiments I need a sodium-free buffer, pH around 7.4
Could anyone suggest me a similar buffer, with the same ionic strenght, but sodium-free?

Thank you!

#2 mdfenko

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Posted 16 April 2013 - 03:46 AM

you could use potassium chloride instead of sodium chloride and potassium hydroxide to adjust the hepes buffer (unless it will cause problems with the cells).
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#3 Annasae

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Posted 16 April 2013 - 04:11 AM

Thank you!!





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