Preparation of Rich Medium for cultivation of Zymomonas sp
Posted 12 April 2013 - 10:28 AM
I need to prepare Rich media containing glucose (RMG) for cultivation of Zymomonas sp.
Composition - Glucose - 2g
KH2PO4:- 0.2 g,
Yeast extract - 1 g,
dissolved in 100 ml distilled water.
I cannot sterilize this media using autoclaving at 121 degrees/15 min. The glucose gets charred.
Does any one have the method for sterilization using syringe filter / pressure cooker?
I do not have a programmable autoclave that can be set at 110 degrees
Posted 12 April 2013 - 11:44 AM
Posted 14 April 2013 - 05:22 PM
Similarly, for fungi, malt extract broth has to be autoclaved only 10 min at 115° C, and believe me, that's important.
How you cannot change the temp in the autoclave? O_o