Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Preparation of Rich Medium for cultivation of Zymomonas sp


  • Please log in to reply
3 replies to this topic

#1 chn09

chn09

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 44 posts
1
Neutral

Posted 12 April 2013 - 10:28 AM

Hello all,

I need to prepare Rich media containing glucose (RMG) for cultivation of Zymomonas sp.

Composition - Glucose - 2g
KH2PO4:- 0.2 g,
Yeast extract - 1 g,
dissolved in 100 ml distilled water.

I cannot sterilize this media using autoclaving at 121 degrees/15 min. The glucose gets charred.

Does any one have the method for sterilization using syringe filter / pressure cooker?

I do not have a programmable autoclave that can be set at 110 degrees

thanks

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,504 posts
253
Excellent

Posted 12 April 2013 - 11:44 AM

You can sterilize the glucose separately, cool, and mix. Alternatively, you can use an 0.22 micron syringe filter to sterilize the solution, or for larger volumes, a bottle top filter.

#3 El Crazy Xabi

El Crazy Xabi

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 199 posts
20
Excellent

Posted 14 April 2013 - 05:22 PM

110° C? Does the recipe states that? Then, do it. It won't only be due the glucose but it will also affect to the YE. Where did you get the recipe from? Are you sure it is the glucose that gets charred? YE-containing media use to get darker after autoclaving and that is in direct relation with the time and temp you autoclave.

Similarly, for fungi, malt extract broth has to be autoclaved only 10 min at 115° C, and believe me, that's important.

How you cannot change the temp in the autoclave? O_o

#4 Phil Geis

Phil Geis

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 219 posts
15
Good

Posted 17 April 2013 - 05:31 AM

You could try the classic technique of Tyndalization.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.