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Determining in frame in modified vector
1 reply to this topic
Posted 12 April 2013 - 06:02 AM
Hi, if I add novel restriction sites to a vector backbone to accept an insert, what is the best way to assess if my vector modification has kept everything in frame? Thanks.
Posted 12 April 2013 - 02:25 PM
If you use multiples of 3 bp for the addition you shouldn't have a problem.