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Deleting restriction enzyme sites


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#1 LacquerHead

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Posted 12 April 2013 - 04:46 AM

Hi,

What is the currently best accepted method to do this in a plasmid? Need to delete a site in the middle of my insert that is crucial for ligating that insert into the vector. Thanks!

Edited by LacquerHead, 12 April 2013 - 04:47 AM.


#2 bob1

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Posted 12 April 2013 - 02:29 PM

Try qickchange PCR or any of the variants.




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