What is the currently best accepted method to do this in a plasmid? Need to delete a site in the middle of my insert that is crucial for ligating that insert into the vector. Thanks!
Edited by LacquerHead, 12 April 2013 - 04:47 AM.
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#1
LacquerHead
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Posted 12 April 2013 - 04:46 AM
Hi,
What is the currently best accepted method to do this in a plasmid? Need to delete a site in the middle of my insert that is crucial for ligating that insert into the vector. Thanks!
What is the currently best accepted method to do this in a plasmid? Need to delete a site in the middle of my insert that is crucial for ligating that insert into the vector. Thanks!
#2
bob1
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Thelymitra pulchella
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Posted 12 April 2013 - 02:29 PM
Try qickchange PCR or any of the variants.
