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Autofluorescence in thick structure cluster of cells


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4 replies to this topic

#1 wdyeo

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Posted 11 April 2013 - 12:18 PM

Hi all,
I am growing heterogenous cells in dishes and my interest is to identify if my cells but these clusters of cells,(thick and round in shape, many layers) do have autofluroscence...
Is there a good fixation method to minimise autofluroscence in the cells which are thick , kind of 3D cluster.
Thank you.

wdyeo

#2 bob1

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Posted 11 April 2013 - 12:22 PM

Try using Bouins fixative (warning -Picric acid!). You can also immerse the samples in 20% DMSO, 20% methanol in PBS or TBS.

#3 wdyeo

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Posted 16 April 2013 - 03:08 AM

Hi bob1,
Sorry for the late reply.
Thanks alot for your suggestion...
But i thought that staining for tissue, no?

#4 bob1

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Posted 16 April 2013 - 05:54 PM

Yes, usually used for tissue, but should be able to use on cells too.

#5 wdyeo

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Posted 17 April 2013 - 02:52 AM

Hi bob1,
Thanks alot for the info :D




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